Nasci launches spermograms by cytometry

One of the most important parameter of the spermogram is spermatozoa concentration.  Why is that? Because the spermogram analysis determines the total number of spermatozoa by multiplying the volume times the concentration. Physicians also use sperm concentration reference values of the World Health Organization (WHO) to decide if low sperm number is the source of the delayed conception and to propose solutions.

The first spermogram method was to observe a small sample under the microscope using a slide that allows the technologist to determine the volume he or she’s covered while counting the spermatozoa.  In this case, the spermogram is done entirely visually and subjectivity can affect the results. Hence such questions as what should be considered the real score if different technologists score the same ejaculate differently.

Facing this, researchers in the 1980’s have eventually developed computer-assisted semen analysis (CASA) methods to perform spermograms. The idea was to bring the image from the microscope to the computer using a camera. Then, a software would do the spermogram by detecting the spermatozoa instead of letting a technologist do it visually. This method eliminates a subjective factor from the spermogram.

Spermograms by CASA are far from perfect for concentration measurements though. In the scientific literature, we often see variations between 25% and 30% (ref, ref) between spermograms performed on the same samples although intensive training and external quality control both together help. Also, some studies tend to show that the use of beads to control the CASA underestimates the real variation that will occur when analysing sperm (ref).

Because every medial laboratory performing spermogram analyses are trying to do the best they can, the debate is on. What is better for a spermogram? Are technologists better than CASA at determining concentration? What is the real concentration when the technologist and the computer don’t agree?

In order to establish itself as a reference in the field of spermogram analysis, Nasci decided to respond to the challenge by introducing cytometry cell counts in fertility spermograms. Cytometry, is a different technology from the ones here previously described to perform spermograms and is mostly used only in research laboratories that perform spermograms.  Cytometry is the gold standard method for cell counts in many medical fields (ref).

Nasci announces that cytometry will be included in all standard fertility spermograms from now on.

This decision is also linked directly to Nasci’s mission to offer state-of-the-art technologies to the patients for a better decision making process that helps them achieve their dream of conceiving.
I wish to thank you sincerely for reading. Other updates on the spermogram at Nasci will keep coming. Visit us often!

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Nasci Biologie Médicale Inc. is actively conducting several research projects on human fertility. Because of that, our laboratory is constantly seeking sperm donors in order to continuously improve the analyses offered and to develop new ones for the future. If you are interested in helping future clients and contributing to the success of our business, please contact us by clicking ici